Device

Part:BBa_J119355:Design

Designed by: Kelly Cochran   Group: Eckdahl Lab   (2014-07-08)


Ammeline Biosensor with Riboswitch D1 + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
    Illegal AgeI site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The D1 riboswitch is based on the M6C" riboswitch with a RBS (AGGAGGT) designed by Davidson College. The sequestration sequence is available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).


Source

M6C"

References